Insertion junction: LMJ.RY0402.181763_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.181763
Chromosome:chromosome 13
Location:691488
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g566350 (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGTTGCAACGATGACAAAGTGGATACCG
Internal bar code:GGGCATAGACGCTGAATAAACC

Confirmation - LEAP-Seq

LEAP-Seq distance:863
LEAP-Seq percent confirming:99.1914
LEAP-Seq n confirming:736
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCGATCACAAGTAACGA
Suggested primer 2:GGTTGAATGCCGAGAAATGT