| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181773 |
| Chromosome: | chromosome 4 |
| Location: | 2367966 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g221000 | (1 of 3) PTHR23024//PTHR23024:SF145 - MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES // ISOPRENYLCYSTEINE ALPHA-CARBONYL METHYLESTERASE ICME-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGAAGCTGTGCGTGTGTACTTCGCTT |
| Internal bar code: | GGCCCCCATAACGCAGACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 916 |
| LEAP-Seq percent confirming: | 99.7206 |
| LEAP-Seq n confirming: | 10350 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTCTTGCGGTTTTGAAGC |
| Suggested primer 2: | GCCGTACCTGTGTCACCTTT |