| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181777 |
| Chromosome: | chromosome 12 |
| Location: | 3391804 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g496950 | (1 of 1) 3.4.13.5 - Xaa-methyl-His dipeptidase / X-methyl-His dipeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGCTCGCTGCTCGAGACGCTGTCGGA |
| Internal bar code: | AAGCGGCACCCGTGAGGGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 982 |
| LEAP-Seq percent confirming: | 98.8314 |
| LEAP-Seq n confirming: | 12770 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 58 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGGGCTAAACGTACTGAAA |
| Suggested primer 2: | CAGCATAGTAGAGGGAGGCG |