| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181805 |
| Chromosome: | chromosome 9 |
| Location: | 1523038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397900 | (1 of 1) PTHR21347:SF0 - CLEFT LIP AND PALATE TRANSMEMBRANE PROTEIN 1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGACCTCTCTAACACCCATTCTGCCGACG |
| Internal bar code: | TTATCGCTTCGGGGTCCCGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1181 |
| LEAP-Seq percent confirming: | 99.3562 |
| LEAP-Seq n confirming: | 1852 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCGAGTTTAGAGCAGTTGG |
| Suggested primer 2: | TCTGCACCCTCTCTCCACTT |