| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181812 |
| Chromosome: | chromosome 11 |
| Location: | 2827827 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g477500 | (1 of 1) PF13664 - Domain of unknown function (DUF4149) (DUF4149) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTATCAATCTTTGCAACCCCCCCTTCCTT |
| Internal bar code: | TCGCGTAATACGGTCCTTATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 46 |
| LEAP-Seq percent confirming: | 98.3425 |
| LEAP-Seq n confirming: | 534 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTTCGCATCTCGTTAGC |
| Suggested primer 2: | TGTCATGTGGTCATGTCGTG |