| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181819 |
| Chromosome: | chromosome 8 |
| Location: | 2244492 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369976 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCGAAAGCGCTGCCAGTCGCGACTCA |
| Internal bar code: | AGAGGAAACGAAGGACGCTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1168 |
| LEAP-Seq percent confirming: | 97.9912 |
| LEAP-Seq n confirming: | 5366 |
| LEAP-Seq n nonconfirming: | 110 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGACCCACCTTGACCAT |
| Suggested primer 2: | TTCACCAACCTGCCTTAACC |