| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181820 |
| Chromosome: | chromosome 3 |
| Location: | 1232474 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149800 | TOG1 | (1 of 1) K16803 - cytoskeleton-associated protein 5 (CKAP5, XMAP215); Microtubule associated protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGTTGCGGATGGTCTCCTCCTCCGAGC |
| Internal bar code: | CGCCCGGCACTGGTACTGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 89.6861 |
| LEAP-Seq n confirming: | 600 |
| LEAP-Seq n nonconfirming: | 69 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGTGGGGTGAAAGAAACG |
| Suggested primer 2: | GAAGTCGCTGATTGAGGAGC |