Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.181821 |
Chromosome: | chromosome_3 |
Location: | 3579066 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g168200 | FAP69 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTAAGTTGACAGGTGCGCCGCTCGCTCATA |
Internal bar code: | TTTCTGCCTGGGTGCAGTTCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 923 |
LEAP-Seq percent confirming: | 99.7474 |
LEAP-Seq n confirming: | 2764 |
LEAP-Seq n nonconfirming: | 7 |
LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGGGGCTAAGCGAGAGGTAG |
Suggested primer 2: | TGCACATGACTAGCAGGAGG |