| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181824 |
| Chromosome: | chromosome 12 |
| Location: | 9340288 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g540650 | (1 of 2) PTHR19241:SF62 - PROTEIN SCARLET | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCACCTGTAGAAGCGGTGGTCGCGCTGC |
| Internal bar code: | ATGAGTTGCGGTAGGGCATGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGGCAAAGTAGACGAAG |
| Suggested primer 2: | GGGTTGCAAGCATGGTTAGT |