| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.181825 |
| Chromosome: | chromosome 7 |
| Location: | 1742661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325716 | DIV146,DNL1,LIG1 | DNA ligase 1; (1 of 1) K10747 - DNA ligase 1 (LIG1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCAGCAACGGAGGTCTACGTTGGTTAGT |
| Internal bar code: | GGCCGACTATGGCCCCGTTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 110 |
| LEAP-Seq percent confirming: | 98.1132 |
| LEAP-Seq n confirming: | 52 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTTTGTACCACCGGACG |
| Suggested primer 2: | AACGGCCACATACAGGACTC |