| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.181826 |
| Chromosome: | chromosome 17 |
| Location: | 2594795 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716900 | (1 of 1) IPR002104//IPR010998//IPR011010 - Integrase, catalytic domain // Integrase, Lambda-type, N-terminal // DNA breaking-rejoining enzyme, catalytic core | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCAGGCAGCCCGCGGCACGCTGGTCAC |
| Internal bar code: | GCCTGGTCGTAGGCTGCATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 97.2353 |
| LEAP-Seq n confirming: | 1442 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCACTGGCACTCCTGCTT |
| Suggested primer 2: | GAGGCTTAAGGCACTTCGTG |