| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181840 |
| Chromosome: | chromosome 1 |
| Location: | 2218038 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g012126 | (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTCTTGCCGTCCAGCAGCGAAAAGGCGC |
| Internal bar code: | AATGCGGAATTGGACGCGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 903 |
| LEAP-Seq percent confirming: | 92.8736 |
| LEAP-Seq n confirming: | 404 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTACTCAGCGAGCGAGGT |
| Suggested primer 2: | TACACTGCAGGAATGAAGCG |