Insertion junction: LMJ.RY0402.181851_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.181851
Chromosome:chromosome 9
Location:7486620
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g413850 GT90-17,GT90F17 GT90 family protein 17; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGACATTGCAAGAAGGGACAGTCCAAGT
Internal bar code:GTCGTTGGGCGCGATATAGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1124
LEAP-Seq percent confirming:99.4075
LEAP-Seq n confirming:5872
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:CGAGCTGTCCAAACAGATGA
Suggested primer 2:ACAGTGCGTGTGTAGCCTTG