Insertion junction: LMJ.RY0402.181854_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.181854
Chromosome:chromosome 9
Location:3662286
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390578 TRP23,FAP11 (1 of 6) PTHR10117 - TRANSIENT RECEPTOR POTENTIAL CHANNEL; Flagellar Associated Protein 11 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGAGACCCCACGCCCATCTACCAGGTGCG
Internal bar code:TGCTATGACATTTGATTTAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1018
LEAP-Seq percent confirming:99.6656
LEAP-Seq n confirming:11623
LEAP-Seq n nonconfirming:39
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:TTAAACAGCAACCAGGACCC
Suggested primer 2:GTTGCAACCACAATGCAATC