Insertion junction: LMJ.RY0402.181922_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.181922
Chromosome:chromosome 14
Location:1076715
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g614850 FAP33,ANK17 Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCGGCGGCTAGCCCCGTCGCCGCCGCCG
Internal bar code:AAAATCAGTTCCCCTTTGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:103
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGATTGTGTGCCGTCTCTC
Suggested primer 2:GTCTGTGTGCTTGAGCGTGT