Insertion junction: LMJ.RY0402.181929_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.181929
Chromosome:chromosome 14
Location:1868207
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g620600 PHC2,FAP202 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACAGTTCAACGTCTACGAGGCGTGCGAC
Internal bar code:TATCAGACCGGATCCTAGAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:98.7981
LEAP-Seq n confirming:411
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGATGGAGTAGGGGAACA
Suggested primer 2:TTCACAGATTTGTCGCTTCG