Insertion junction: LMJ.RY0402.181929_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.181929
Chromosome:chromosome 14
Location:1868421
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g620600 PHC2,FAP202 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACATCACCCAGCTTGTAGGCCGAGGGAGA
Internal bar code:GGTGAGCAAGAGTGTATACCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:322
LEAP-Seq percent confirming:92.6561
LEAP-Seq n confirming:757
LEAP-Seq n nonconfirming:60
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACAGATTTGTCGCTTCG
Suggested primer 2:GCTGATGGAGTAGGGGAACA