| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.181954 |
| Chromosome: | chromosome 12 |
| Location: | 6385656 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g537611 | Class II-Zygote-specific protein with GPI-anchor signal | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGCCACCTCATGCCACCTCGTCGCGTC |
| Internal bar code: | TCTCTCTTGATATGGACCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 98.7277 |
| LEAP-Seq n confirming: | 388 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTCACCCGCTTCCATTC |
| Suggested primer 2: | CACGATATCTACGCGCTTCA |