Insertion junction: LMJ.RY0402.182023_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.182023
Chromosome:chromosome 1
Location:4005862
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g025983 protein of unknown function with DUF1399 domain; (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGTGCCGTGGGCCCCGTACGACCAGCC
Internal bar code:CATGTAATGTGTCGTTTCTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:373
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:266
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACAGCTTACTCCAAAGC
Suggested primer 2:ATCACCACGTCCATGTCAGA