| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182023 |
| Chromosome: | chromosome_10 |
| Location: | 5789307 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g461350 | BUG23 | Basal body protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTAAACGGAGGGGGGGCTGCGGAGAAGCCT |
| Internal bar code: | CCGCCATAAGCCGAGAGGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.6262 |
| LEAP-Seq n confirming: | 2399 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTATGAGGCTGAGCTGCTT |
| Suggested primer 2: | ACAGCCAGAGCGAAATCTGT |