| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182023 |
| Chromosome: | chromosome_12 |
| Location: | 5089223 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g526700 | RBL9 | Rhomboid-like protease | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGACACACACGCACAACTGAGTATCCTCA |
| Internal bar code: | GCGGGTAGCTGTAGGTGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.8186 |
| LEAP-Seq n confirming: | 3853 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTGTGTGTGGGTGTGTG |
| Suggested primer 2: | CAGGCTGTGTGCTGTTTTGT |