| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182025 |
| Chromosome: | chromosome 3 |
| Location: | 3105024 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g164800 | (1 of 1) PTHR24361:SF363 - PROTEIN KINASE-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCCCCGCCCCCCGCCCTCAAGAGTTGT |
| Internal bar code: | GGTGGCTGGGACAGCGGTCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 295 |
| LEAP-Seq percent confirming: | 99.8652 |
| LEAP-Seq n confirming: | 741 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGATCTCCTCCAGCTCGT |
| Suggested primer 2: | TGAGTTCCCTTCGGATAACG |