| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182050 |
| Chromosome: | chromosome 4 |
| Location: | 2688301 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCTGGAAGCCACGTGCCAGGCTGGCCCC |
| Internal bar code: | GGCCGGGAGGGCGTGTATAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 988 |
| LEAP-Seq percent confirming: | 87.6015 |
| LEAP-Seq n confirming: | 4854 |
| LEAP-Seq n nonconfirming: | 687 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAAAGCACCGTCGTCATCC |
| Suggested primer 2: | CCCGAGTACTCCATCCTCAA |