| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182058 |
| Chromosome: | chromosome 2 |
| Location: | 7083725 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147050 | (1 of 7) PF05517 - p25-alpha (p25-alpha); P25 Alpha Domain Containing Flagellar Associated Protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAACGCATATGCCCGTAGACCTGAGGAAG |
| Internal bar code: | GTTGGTGGCAAAAAACTCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 99.1216 |
| LEAP-Seq n confirming: | 2144 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACATGTGGATCGTGAG |
| Suggested primer 2: | CAGCATTTGTTGGTGTGGAC |