| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182060 |
| Chromosome: | chromosome 7 |
| Location: | 3933266 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339250 | (1 of 1) K12611 - mRNA-decapping enzyme 1B (DCP1B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGATGAGGGGTTGGCCGTGGGGTTTAGAA |
| Internal bar code: | TCTGCGGAGAGCACGCGGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1046 |
| LEAP-Seq percent confirming: | 98.586 |
| LEAP-Seq n confirming: | 4532 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGGGATAAGCCAGTCACC |
| Suggested primer 2: | TGTACTCTTCCCCCAGTTCG |