Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.182088 |
Chromosome: | chromosome_16 |
Location: | 2598641 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g661638 | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATTTGGGCTTAATGGTTCAAGGACATGGGA |
Internal bar code: | AAGGACGACTGGAGCGAGAAAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 979 |
LEAP-Seq percent confirming: | 99.3786 |
LEAP-Seq n confirming: | 1919 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACGTGCTTCCTGCAACAAC |
Suggested primer 2: | GCTACAGTCACCATTCCCGT |