| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182107 |
| Chromosome: | chromosome 3 |
| Location: | 5100977 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g181450 | TCTN1,POB7 | (1 of 1) K19382 - tectonic-1/3 (TCTN1_3); Proteome of basal body 7 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGTGATGTTGGCGCCGTATGCCACGTTG |
| Internal bar code: | GCCGCGGCCGGCATGATAGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1016 |
| LEAP-Seq percent confirming: | 97.9567 |
| LEAP-Seq n confirming: | 815 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAAAGCAGCATGCCGTAG |
| Suggested primer 2: | AGCACCTATAACGCCACCAC |