| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182110 |
| Chromosome: | chromosome 1 |
| Location: | 6941145 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g050000 | (1 of 1) PTHR31204//PTHR31204:SF1 - FAMILY NOT NAMED // TRANSMEMBRANE PROTEIN 97 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGATAACTCCCCCTCAATCTGAGCACCGG |
| Internal bar code: | AGTTTGCAGGGTGACACGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 841 |
| LEAP-Seq percent confirming: | 75.5172 |
| LEAP-Seq n confirming: | 1314 |
| LEAP-Seq n nonconfirming: | 426 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTTGTGGCTTTTAACGA |
| Suggested primer 2: | GCAAGGTGTTGGAGGTGTTT |