| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182120 |
| Chromosome: | chromosome 7 |
| Location: | 3398771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335200 | GBA1,EFG12 | (1 of 1) PTHR23115//PTHR23115:SF165 - TRANSLATION FACTOR // TYPA-LIKE TRANSLATION ELONGATION FACTOR SVR3-RELATED; Putative chloroplast TypA translation elongation GTPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGTTTCTGGTGGTTTCGTTTGTGGTCCA |
| Internal bar code: | TGTATTTGTAGCTCAAGACAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 831 |
| LEAP-Seq percent confirming: | 66.2634 |
| LEAP-Seq n confirming: | 6079 |
| LEAP-Seq n nonconfirming: | 3095 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGAACCCATCAATTTCT |
| Suggested primer 2: | ATCAAGTTCCGTATCGCCAC |