| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182125 |
| Chromosome: | chromosome_9 |
| Location: | 1267695 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g399400 | FAP199,TGL15 | Triacylglycerol lipase | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGTTGGTGTGTGTGTGTGTGTGTGCGTGT |
| Internal bar code: | ATGTAATACAGTGCCAGCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 0.18432 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 32492 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACTGATCAGGAAACCCC |
| Suggested primer 2: | TTTGGGATATCATGGACCGT |