| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182128 |
| Chromosome: | chromosome 1 |
| Location: | 30337 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g000050 | RWP14 | (1 of 16) PF02042 - RWP-RK domain (RWP-RK); RWP-RK transcription factor | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCATCCATCTGGCTTTCATTTTTTGTC |
| Internal bar code: | GACAAATCTGTTTTCACAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1059 |
| LEAP-Seq percent confirming: | 99.5499 |
| LEAP-Seq n confirming: | 16367 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGAGCTTCGACGTAAAC |
| Suggested primer 2: | GCCCGAGCAAGTTCTAGTTG |