| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182148 |
| Chromosome: | chromosome_6 |
| Location: | 1195483 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g257600 | FA1 | Flagellar Autonomy Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGGTGCGCTCCCTCACTGCTGCTAATTCA |
| Internal bar code: | TTGGGTTGAGATAGTCAAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 162 |
| LEAP-Seq percent confirming: | 34.1431 |
| LEAP-Seq n confirming: | 10218 |
| LEAP-Seq n nonconfirming: | 19709 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATAGATGGTTGCAAGGGGA |
| Suggested primer 2: | TAGCTTGACGCACACACACA |