| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182185 |
| Chromosome: | chromosome 10 |
| Location: | 530385 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421250 | EXO70 | (1 of 1) K07195 - exocyst complex component 7 (EXOC7, EXO70); Component of the Exocyst Complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGCGGGTGACGGTCTGAGGAAGCGGTG |
| Internal bar code: | GCTGCGTCGACGCTACCACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1021 |
| LEAP-Seq percent confirming: | 99.4145 |
| LEAP-Seq n confirming: | 5603 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCGTGATTGTGTACCCT |
| Suggested primer 2: | TCACAAGCGACAAGTCCAAG |