Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.182208 |
Chromosome: | chromosome 1 |
Location: | 4604667 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre01.g031650 | CGLD12 | Conserved in the Green Lineage and Diatoms; (1 of 2) PF05637 - galactosyl transferase GMA12/MNN10 family (Glyco_transf_34) | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCTCACGCGCGTGACGTCGTACTACACA |
Internal bar code: | CGGTCGTGCCGTTCATTTGGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 937 |
LEAP-Seq percent confirming: | 74.1452 |
LEAP-Seq n confirming: | 1583 |
LEAP-Seq n nonconfirming: | 552 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAGGGACATCACTAATGCCG |
Suggested primer 2: | CACATGAACGGTGAAGGTTG |