Insertion junction: LMJ.RY0402.182208_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.182208
Chromosome:chromosome 1
Location:4604667
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g031650 CGLD12 Conserved in the Green Lineage and Diatoms; (1 of 2) PF05637 - galactosyl transferase GMA12/MNN10 family (Glyco_transf_34) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCTCACGCGCGTGACGTCGTACTACACA
Internal bar code:CGGTCGTGCCGTTCATTTGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:937
LEAP-Seq percent confirming:74.1452
LEAP-Seq n confirming:1583
LEAP-Seq n nonconfirming:552
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGGACATCACTAATGCCG
Suggested primer 2:CACATGAACGGTGAAGGTTG