| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182209 |
| Chromosome: | chromosome 6 |
| Location: | 1326790 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGTGATACGAGCTCGTACGAGGAGGTT |
| Internal bar code: | TCATTCCCTGCCAGGATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 76.6923 |
| LEAP-Seq n confirming: | 1099 |
| LEAP-Seq n nonconfirming: | 334 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGTATCTAGCTGCTGTTG |
| Suggested primer 2: | GACATGCTGGTAGCTTGGGT |