Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.182215 |
Chromosome: | chromosome 4 |
Location: | 1753457 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre04.g212250 | (1 of 82) IPR020846 - Major facilitator superfamily domain; GT77 family protein | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTGAGTCCCCGAGTCCCTGAGTCACTG |
Internal bar code: | TTTGTCCTTTTTACCCCAAGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 194 |
LEAP-Seq percent confirming: | 94.5573 |
LEAP-Seq n confirming: | 4795 |
LEAP-Seq n nonconfirming: | 276 |
LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CATCTCTCCCCTCTATCCCC |
Suggested primer 2: | CAGGGGGTTACACTCATGCT |