Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.182223 |
Chromosome: | chromosome 10 |
Location: | 2610177 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre10.g437300 | (1 of 1) PF00053 - Laminin EGF domain (Laminin_EGF) | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATGTGAGCCGCAGCCGCAGCGGGTCGCC |
Internal bar code: | TATAGGTCTTTGTTCCTCTTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 731 |
LEAP-Seq percent confirming: | 95.6311 |
LEAP-Seq n confirming: | 394 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACCTGAGGAGCCTTGAGAC |
Suggested primer 2: | ACAGCTTGGTGTAGTTGGGG |