| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182223 |
| Chromosome: | chromosome 10 |
| Location: | 2610181 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437300 | (1 of 1) PF00053 - Laminin EGF domain (Laminin_EGF) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGCCGCCCAGGTGCGTGCGTGTCAATG |
| Internal bar code: | ACCGGAGGGGGGGGAGGAGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 800 |
| LEAP-Seq percent confirming: | 99.5738 |
| LEAP-Seq n confirming: | 6542 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGAGGAGCCTTGAGAC |
| Suggested primer 2: | ACAGCTTGGTGTAGTTGGGG |