| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182231 |
| Chromosome: | chromosome 10 |
| Location: | 1223745 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426550 | (1 of 1) IPR003347//IPR006917 - JmjC domain // SOUL haem-binding protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACGTCTCCAATTTGCTACCTCTTCCTG |
| Internal bar code: | GCGCTGCCCTCAGGCTCTGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 431 |
| LEAP-Seq percent confirming: | 99.7404 |
| LEAP-Seq n confirming: | 4611 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTAGCCAACCGCGACAT |
| Suggested primer 2: | TCGCTGCCACATATTCTTTG |