| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182239 |
| Chromosome: | chromosome 3 |
| Location: | 7558805 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205000 | IDA3 | Inner Arm Adaptor to IFT 3; (1 of 1) PF15669 - Coiled-coil domain-containing protein 24 family (CCDC24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCCCTAGCAGCAGGGCCCCAACCAAC |
| Internal bar code: | CTTGTGCCGTGAAACCGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 235 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGCGTCATTGTGTGTC |
| Suggested primer 2: | CTCTGCGAGAGTCCCGTATC |