| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182245 |
| Chromosome: | chromosome 3 |
| Location: | 6198533 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g192300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGCTGCCACCTCCCTGTGCCGCTGTCT |
| Internal bar code: | CGGGACGGTTCTTTGGTGGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 84.5902 |
| LEAP-Seq n confirming: | 1290 |
| LEAP-Seq n nonconfirming: | 235 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCTCCTTGTGCAATCTC |
| Suggested primer 2: | CCTCGTAAGCACCCACAAAT |