| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182256 |
| Chromosome: | chromosome 3 |
| Location: | 6725748 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197800 | (1 of 3) PF01843 - DIL domain (DIL) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCTGCATTAGGGTGCCAAGCAGCGGAG |
| Internal bar code: | CAGCACCGGCGGTGGCACGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 99.5187 |
| LEAP-Seq n confirming: | 3929 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCGAGACTATGCCTGC |
| Suggested primer 2: | TGCACCCTACGGGACTTAAC |