Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.182282 |
Chromosome: | chromosome_4 |
Location: | 1934132 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g218250 | ARL3 | ARF-like GTPase | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGGACATTGGCGGCCAGAAGTCCATCCGC |
Internal bar code: | CACGGCTGCGGCCTCTTCTCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 195 |
LEAP-Seq percent confirming: | 99.7293 |
LEAP-Seq n confirming: | 4052 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGCTCCTCGCCTCACTAAA |
Suggested primer 2: | GAAGAAGCGTTCGGTTGAAG |