| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182285 |
| Chromosome: | chromosome 3 |
| Location: | 1503783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g151950 | LZY1B | Zygote-specific Cys-rich small protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGCGACAGAGCCGCGCCCAGCCATCAA |
| Internal bar code: | TGAAGGTCGAGTAAACTTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1092 |
| LEAP-Seq percent confirming: | 97.5328 |
| LEAP-Seq n confirming: | 3716 |
| LEAP-Seq n nonconfirming: | 94 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGGATTACAGGTGCGT |
| Suggested primer 2: | GCCTTCGCTTCGTCTATGTC |