| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182301 |
| Chromosome: | chromosome 1 |
| Location: | 6163835 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044100 | AMYB3,AMB3 | Beta-amylase 3; (1 of 3) 3.2.1.2 - Beta-amylase / Saccharogen amylase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAAGAGGAACGGTTTTGTGCAAAGGG |
| Internal bar code: | TTTCGCGAGAGCTCGGCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 96.2698 |
| LEAP-Seq n confirming: | 3897 |
| LEAP-Seq n nonconfirming: | 151 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGGTGGGAGATGTATGC |
| Suggested primer 2: | GGTATGTGCATGTCTGGTGC |