| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182305 |
| Chromosome: | chromosome 14 |
| Location: | 1299582 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616750 | PEX5 | Peroxisomal targeting signal 1 receptor; (1 of 1) K13342 - peroxin-5 (PEX5, PXR1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTACTCAATCAGTCACTTACGTGCTCTG |
| Internal bar code: | GGGGACTACTAAAGTCAGTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 99.5841 |
| LEAP-Seq n confirming: | 4789 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATGTGCGGTGTATGTC |
| Suggested primer 2: | GCAAGCTAGAACTGATGGGC |