| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182320 |
| Chromosome: | chromosome 7 |
| Location: | 4947435 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346550 | (1 of 8) 2.7.11.1//2.7.12.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase // Dual-specificity kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGTCTGCGACCATTTTGAAAATCACAGG |
| Internal bar code: | ACCAAGCGGCACGTTTCTGGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1075 |
| LEAP-Seq percent confirming: | 99.4184 |
| LEAP-Seq n confirming: | 11283 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 79 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGGAAGTAGCCGACAG |
| Suggested primer 2: | CGCAGCAATTGGGTTTATTT |