| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182333 |
| Chromosome: | chromosome 16 |
| Location: | 5534335 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGAGAGCACCCCGCGGTAACCCCCCGC |
| Internal bar code: | CGGTTGATTCGGCGATGGTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 581 |
| LEAP-Seq percent confirming: | 51.5385 |
| LEAP-Seq n confirming: | 469 |
| LEAP-Seq n nonconfirming: | 441 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAACCAACAACCGTCCTT |
| Suggested primer 2: | TCTACCTATGCTCCGCTCGT |