| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182334 |
| Chromosome: | chromosome 8 |
| Location: | 3620227 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376150 | CGL150 | (1 of 1) K17866 - diphthamide biosynthesis protein 2 (DPH2); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGGGAACAGGATACAAGCAGGGGAAGAA |
| Internal bar code: | TGCTCGTGTTCTGCAGAGAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 556 |
| LEAP-Seq percent confirming: | 99.6208 |
| LEAP-Seq n confirming: | 3678 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGGTGTTGCAGATGAC |
| Suggested primer 2: | AATACGTCCACCTGCGAAAC |