| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182336 |
| Chromosome: | chromosome 17 |
| Location: | 867415 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g702451 | HLM27 | putative N-methyltransferase; (1 of 1) PTHR22884:SF348 - PROTEIN SET-25 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTAGCTGCCTGCGGCTGAAACACACAC |
| Internal bar code: | TCGGTGACGAGAGATAACTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 72 |
| LEAP-Seq percent confirming: | 37.5 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCGTTTCCTGAACGATGT |
| Suggested primer 2: | TATGGTAGCAGCAGTGGCAG |